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This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. Synonyms: Isopropyl β-D-thiogalactoside IPTG Formula: C 9H 18O 5S Molecular Weight: 238.3 g/mol General information No special measures required. If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult doctor in case of complaints. In case of skin contact Immediately wash off with soap and The plasmid has the insert for the protein with His tag and the expected molecular weight is around 50 kDa. If you receive a signal at 50/51 kDa probing with an anti-His antibody, don't you think The molecular mass of the recombinant ACTH was confirmed by mass spectrometry to equal 4567.32 Da.

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Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water. Concentration buffer exchange unit with low-molecular-weight (MW) cutoff (e.g., Centricon) (optional; see Step 24) Ice. Incubator (shaking), preset to 37°C. Microcentrifuge and microcentrifuge tubes. Rotator for end-over-end mixing. Commercially available glutathione-Sepharose beads are often provided in a solution containing alcohols or other Bacterial containing pET-SUMO-FGF21 were induced by IPTG for 3 h or 4 h at 37°C in Figure 3A. The results from the different treatments are shown here.

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Tris base. Coomassie protein gel stain CAS: 367-93-1: Molecular Formula: C9H18O5S: Molecular Weight (g/mol) 238.298: InChI Key: BPHPUYQFMNQIOC-NXRLNHOXSA-N: Synonym: iptg, isopropyl-beta-d-thiogalactopyranoside, isopropyl-beta-d-thiogalactoside, isopropyl thiogalactoside, isopropyl beta-d-thiogalactopyranoside, isopropyl beta-d-thiogalactoside, isopropyl beta-d-1-thiogalactopyranoside, unii-x73vv2246b, 2r,3r,4s,5r,6s-2 Description. IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where … 2021-03-09 2011-12-23 IPTG (Isopropyl β-D-thiogalactoside, Isopropyl thiogalactoside, Isopropyl β-D-1-thiogalactopyranoside, lad-y) is a galactose and a molecular mimic of allolactose that triggers transcription of the lac operon, stimulating protein expression.

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Beilstein/REAXYS Number 4631 . EC Number 206-703-0. MDL number MFCD00063273. PubChem Substance ID 24896093. NACRES NA.31 Molecular Weight: 238.3 g/mol: Computed by PubChem 2.1 (PubChem release 2019.06.18) XLogP3-AA-0.5: Computed by XLogP3 3.0 (PubChem release 2019.06.18) Hydrogen Bond Donor Count: 4: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Hydrogen Bond Acceptor Count: 6: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Rotatable Bond Count: 3 Synonym: IPTG, Dioxane-Free, High Purity - CAS 367-93-1 - Calbiochem, Isopropyl-β-D-thiogalactopyranoside CAS Number 367-93-1. Empirical Formula (Hill Notation) C 9 H 18 O 5 S .

Technical info. MSDS. Certificate of Analysis. Isopropyl-ß-D-thiogalactoside, IPTG is a chemical analogue of galactose that cannot be cleaved by ß-galactosidase. IPTG is therefore able to induce protein expression where the gene is under the control of the lac operator, when used as an effective inducer in the concentration range of 100μM to 1.5mM. Features >99% Purity; Synonym: IPTG, Isopropyl β-D-thiogalactoside; CAS#: 367-93-1; Molecular Formula: C 9 H 18 O 5 S; Molecular Weight: 238.3; Applications. Blue/white screening Protein Molecular Weight Marker.
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Product Code: EI05931. MDL No: MFCD00063273. Chemical Formula: C9H18O5S. Molecular Weight: 238.3 g/mol   Molecular Formula: C9H18O5S. Molecular Weight: 238.3.

Chemical Formula: C9H18O5S. Solubility: Water, ethanol. Storage Temperature: 2-8°C. IPTG is combined with X-GAL to detect lac+  The following data is based on the product molecular weight 238.3.
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dH2O, 0.8 ml  Synonyms: IPTG non-animal origin;IPTG. Product Code: EI05931. MDL No: MFCD00063273. Chemical Formula: C9H18O5S.

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Note: To prepare 10 mL 1M IPTG stock solution, weight 2.38 g IPTG and dissolve in 8 ml distilled water, adjust to a final volume of 10 ml with distilled water.

Chemical Formula: C 9 H 18 O 5 S. Harmonized Code: 2932990090. IPTG is combined with X-GAL to detect lac+ colonies or cells in a 2015-01-01 · IPTG stock solution (1 Lane 1, molecular weight markers. Lane 2, MEX67 un-induced control. Lane 3, MEX67 induced in presence of 100 IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. Activity. T7 polymerase is extremely promoter-specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a double stranded DNA template and Mg 2+ ion as cofactor for the synthesis of RNA. Synonyms: Isopropyl β-D-thiogalactoside IPTG Formula: C 9H 18O 5S Molecular Weight: 238.3 g/mol General information No special measures required.